| 质粒名称: | ND6-DdCBE-left side TALE |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 157842 |
| 存储实验室: | David Liu |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 载体出品公司: | Clontech |
| 空载体大小: | 7214 bp |
| 载体类型: | 哺乳细胞表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Mach1 |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | SOD2 MTS–3x-HA–ND6 left tale–G1397 DddA-N–UGI–SOD2 3'UTR |
|
文献引用方法 |
|
| 材料和方法部分: | ND6-DdCBE-left side TALE was a gift from David Liu (Addgene plasmid # 157842 ; http://n2t.net/addgene:157842 ; RRID:Addgene_157842) |
| 参考文献部分: | A bacterial cytidine deaminase toxin enables CRISPR-free mitochondrial base editing. Mok BY, de Moraes MH, Zeng J, Bosch DE, Kotrys AV, Raguram A, Hsu F, Radey MC, Peterson SB, Mootha VK, Mougous JD, Liu DR. Nature. 2020 Jul 8. pii: 10.1038/s41586-020-2477-4. doi: 10.1038/s41586-020-2477-4. 10.1038/s41586-020-2477-4 PubMed 32641830 |
买家导航
ND6-DdCBE-left side TALE载体质粒基本信息
ND6-DdCBE-left side TALE质粒图谱载体图谱和ND6-DdCBE-left side TALE载体序列质粒序列多克隆位点信息
ND6-DdCBE-left side TALE质粒载体简介
ND6-DdCBE-left side TALE质粒序列载体序列
LOCUS ND6-DdCBE-left side TALE 7214 bp DNA circular SYN 14-APR-2025
DEFINITION expresses left-side TALE–DddAtox half of ND6-DdCBE in mammalian
cells.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7214)
AUTHORS Mok BY, de Moraes MH, Zeng J, Bosch DE, Kotrys AV, Raguram A, Hsu F,
Radey MC, Peterson SB, Mootha VK, Mougous JD, Liu DR
TITLE A bacterial cytidine deaminase toxin enables CRISPR-free
mitochondrial base editing.
JOURNAL Nature. 2020 Jul 8. pii: 10.1038/s41586-020-2477-4. doi:
10.1038/s41586-020-2477-4.
PUBMED 32641830
REFERENCE 2 (bases 1 to 7214)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nature.
2020 Jul 8. pii: 10.1038/s41586-020-2477-4. doi:
10.1038/s41586-020-2477-4."
COMMENT Sequence Label: ND6-DdCBE-left side TALE
FEATURES Location/Qualifiers
source 1..7214
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind complement(44..63)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 235..614
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 769..789
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 863..882
/label=T7
/note="T7 promoter, forward primer"
promoter 863..881
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 997..1023
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 1027..1053
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 1057..1083
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 2944..3195
/codon_start=1
/product="uracil-DNA glycosylase inhibitor from a Bacillus
subtilis bacteriophage (Mol et al., 1995)"
/label=UGI
/translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
rep_origin 3446..3874
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(3533..3552)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 3743..3764
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(3883..3903)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 3888..4217
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 4068..4203
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 4130..4149
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
promoter 4265..4312
/label=EM7 promoter
/note="synthetic bacterial promoter "
CDS 4331..4729
/codon_start=1
/gene="Aspergillus terreus BSD"
/product="blasticidin S deaminase"
/label=BSD
/note="confers resistance to blasticidin"
/translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
KAIVKDSDGQPTAVGIRELLPSGYVWEG"
polyA_signal 4887..5008
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(4924..4943)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 4978..4997
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(5057..5073)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5057..5073)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(5070..5092)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 5081..5097
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(join(5105..5111,5112..5129,5130..5135))
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5150..5171
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(5288..5305)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(5459..6047)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5539..5558)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(join(6218..7009,7010..7078))
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 6841..6860
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(7079..7183)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 accatggccc tgtcccgtgc ggtttgtggc acctcccgtc aactggctcc ggttctgggt
961 tatctgggtt cccgtcaaaa acactccctg ccggactacc cgtatgatgt tccggattac
1021 gctggctacc catacgacgt cccagactac gctggctacc catacgacgt cccagactac
1081 gctatggaca tcgcggatct gcgtaccctg ggttacagcc agcagcagca ggagaagatc
1141 aagccgaagg tgcgcagcac cgtggctcag caccacgaag ccctggtggg ccacggtttc
1201 acccacgctc acattgtggc cctgagccag cacccagccg cgctgggcac cgtggccgtg
1261 aaatatcagg atatgattgc tgccctgcca gaggccaccc atgaagctat tgtgggcgtg
1321 ggcaagcagt ggagcggtgc tcgtgcgctg gaggcgctgc tgaccgtggc tggtgaactg
1381 cgtggtccgc cgctgcagct ggacaccggt cagctgctga aaatcgcgaa acgtggcggt
1441 gtgaccgcgg tggaagccgt gcatgcttgg cgtaatgctc tgaccggtgc gccgctgaac
1501 ctgaccccgc agcaggtggt ggctattgcc agcaacaacg gcggtaaaca ggccctggag
1561 accgtgcagc gcctgctgcc ggtgctgtgc caggcccatg gtctgacccc ggagcaggtg
1621 gtggcgatcg ctagcaacat cggcggcaag caggccctgg aaaccgtgca ggcgctgtta
1681 ccggtgctgt gccaggctca tggcctgacc ccggaacaag ttgtggctat tgccagccat
1741 gatggcggta aacaggctct ggaaaccgtg cagcgtctgt tgccggtgct gtgccaagcc
1801 catggcctga ccccggagca agttgtggct attgcgagcc atgatggcgg caagcaggcg
1861 ctggaaaccg ttcagcgcct gttaccggtg ctgtgccaag ctcatggtct gaccccggaa
1921 caggtggtgg ccattgcttc ccatgatggc ggtaaacagg ccctggaaac cgttcagcgt
1981 ctgcttccgg tgctgtgcca ggcccatggg ctgaccccgg aacaagtggt tgctattgcc
2041 agccacgatg gcggcaagca ggctctggag accgttcagc gcctgcttcc ggtgctgtgc
2101 caggcccatg gcttaacccc ggaacaagtt gttgctattg ctagtcatga tggcggtaaa
2161 caggcgctgg agaccgttca gcgtctgtta ccggtgctgt gccaggcgca tggcttaacc
2221 ccggagcagg ttgttgccat tgcctccaat atcggcggca agcaggctct ggaaaccgtt
2281 caggccctgt tgccggtgct gtgccaggcc catggactga ccccgcagca agttgttgcc
2341 attgccagca atggcggtgg caaacaggcg ctggaaactg ttcagcgcct gctcccggtg
2401 ctgtgccaag cgcatggtct gaccccgcag caagtggttg ctattgctag caatggtggc
2461 ggtcgtccgg cgctggaaag cattgtggct cagctgagcc gtccagaccc ggccctggcg
2521 gctctgacca acgatcacct ggtggcgctg gcttgcctgg gcggtcgtcc ggccctggat
2581 gcggtgaaga aaggcctggg tggatccggc agctacgccc tgggtccgta tcagattagc
2641 gccccgcagc tgccagctta caatggtcag accgtgggta ccttctacta tgtgaacgac
2701 gcgggcggtc tggagagcaa ggtgtttagc agcggcggtc caaccccgta cccaaactat
2761 gccaatgccg gtcatgtgga gggtcagagc gccctgttca tgcgtgataa cggcatcagc
2821 gagggtctgg tgttccacaa caacccggaa ggcacctgcg gtttttgcgt gaacatgacc
2881 gagaccctgc tgccggaaaa cgcgaaaatg accgtggtgc cgccggaagg ttctggcggc
2941 tcaactaatc tgagcgacat cattgagaag gagactggga aacagctggt cattcaggag
3001 tccatcctga tgctgcctga ggaggtggag gaagtgatcg gcaacaagcc agagtctgac
3061 atcctggtgc acaccgccta cgacgagtcc acagatgaga atgtgatgct gctgacctct
3121 gacgcccccg agtataagcc ttgggccctg gtcatccagg attctaacgg cgagaataag
3181 atcaagatgc tgtgataaac cacgatcgtt atgctgatca taccctaatg atcccagcaa
3241 gataatgtcc tgtcttctaa gatgtgcatc aagcctggta catactgaaa accctataag
3301 gtcctggata atttttgttt gattattcat tgaagaaaca tttattttcc aattgtgtga
3361 agtttttgac tgttaataaa agaatctgtc aaccatcaaa cttctgaggc ggaaagaacc
3421 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
3481 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
3541 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
3601 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
3661 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
3721 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
3781 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
3841 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag
3901 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
3961 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
4021 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
4081 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
4141 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag
4201 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc
4261 acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag
4321 gaactaaacc atggccaagc ctttgtctca agaagaatcc accctcattg aaagagcaac
4381 ggctacaatc aacagcatcc ccatctctga agactacagc gtcgccagcg cagctctctc
4441 tagcgacggc cgcatcttca ctggtgtcaa tgtatatcat tttactgggg gaccttgtgc
4501 agaactcgtg gtgctgggca ctgctgctgc tgcggcagct ggcaacctga cttgtatcgt
4561 cgcgatcgga aatgagaaca ggggcatctt gagcccctgc ggacggtgcc gacaggtgct
4621 tctcgatctg catcctggga tcaaagccat agtgaaggac agtgatggac agccgacggc
4681 agttgggatt cgtgaattgc tgccctctgg ttatgtgtgg gagggctaag cacttcgtgg
4741 ccgaggagca ggactgacac gtgctacgag atttcgattc caccgccgcc ttctatgaaa
4801 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
4861 tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat
4921 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
4981 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga
5041 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
5101 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
5161 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
5221 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
5281 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
5341 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
5401 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
5461 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
5521 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
5581 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
5641 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
5701 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
5761 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
5821 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
5881 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
5941 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
6001 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
6061 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
6121 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
6181 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
6241 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
6301 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
6361 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
6421 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
6481 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
6541 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
6601 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
6661 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
6721 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
6781 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
6841 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
6901 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
6961 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
7021 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
7081 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
7141 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
7201 tgccacctga cgtc
//
